Thick Brain Slice Cultures and a Custom-fabricated Multiphoton Imaging System: Progress towards Development of a 3d Hybrot Model
نویسندگان
چکیده
Today, fluorescence imaging spans wide spectrum in modern biological investigations from molecular to network level at submicron resolution. To take advantage of full potential of advanced fluorescence imaging techniques, it is important to understand some underlying physical principles. This chapter provides a framework to understand basic principles underlying fluorescence, fluorescence microscopy, one-photon and multi-photon laser-scanning microscopy, and advantages of multiphoton imaging of live tissue over single-photon imaging.
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